文献 | 量子荧光|微型光纤光谱仪-ag贵宾会

double detection of microsphere light scattering and quantum dot emission was demonstrated for lab-on-a-chip immunoassay without using stationary support. we conjugated quantum dots (qds) onto microspheres to enable multiplex assays as well as to enhance the limit of detection (lod). we named this configuration “nano-on-micro” or “nom”. upon radiation with uv light (380 nm), a stronger light scattering signal is observed with noms than qds or microspheres alone. additionally, noms are easier to handle than qds. since qds also provide fluorescent emission, we are able to utilize an increase in light scattering for detecting antigen–antibody reaction and a decrease in qd emission to identify which antibody (or antigen) is present. two types of nom combinations were used. one batch of microspheres was coated with qds emitting at 655 nm and mouse igg (migg); the other with qds emitting at 605 nm and bovine serum albumin (bsa). a mixture of these two noms was used to identify either anti-migg or anti-bsa. nom particles and target solutions were mixed in a microfluidic device (using highly carboxylated microspheres as previously demonstrated by our group) and on-chip detectionwas performed using proximity optical fibers. forward light scattering at 380 nm was collected. with the positive target, the scattering signal was increased. the lod was as low as 50 ng ml?1 (330 pm) with p < 0.05. fluorescent emission (655 or 605 nm) was simultaneously collected. with the positive target, the emission signal was attenuated. therefore, we were able to detect two different antibodies simultaneously with two different detection protocols. we believe this nom bioassay has the ability to screen for and detect multiple antibodies with minimal sample processing and handling (one-step lab-on-a-chip immunoassay).

background. there is a lack of data on polymerization of resin-based materials (rbms) used in paediatric dentistry, using dual-peak light-emitting diode (led) light-curing units (lcus). objective. to evaluate the degree of conversion (dc) of rbms cured with dual-peak or singlepeak led lcus.

我国是水果生产大国，却是水果出口小国。水果商品化处理程度低是导致我国水果国际市场竞争力弱、出口量小的主要原因之一，提高我国的水果商品化处理水平能有效地增强我国水果的国际市场竞争力，扩大我国水果出口量，水果外观和内部品质的快速无损检测是水果商品化处理的重要环节，因而，实现水果外观和内部品质的快速无损检测是非常重要和必要的。

报道了一种蓝光(471nm)抽运的全固态pr∶kyf激光器，其入射抽运激光阈值功率为0.4w,当注入的抽运功率为2.5w 时,得到了最大212mw 的610nm波长的激光输出。然后采用i类临界相位匹配的β-bab2o4(bbo)晶体进行内腔倍频,得到了最大11mw 的二次谐波305nm紫外激光输出，通过刀口法可测量到在tem00模式下,输出光束的质量因子m2=1.25;光-光转换效率达4.4%;输出功率在30min内的不稳定度优于4.3%。采用usb-4000型光谱仪检测到输出为305nm的激光光谱。